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CHAPTER ONE INTRODUCTION1

Section 1 The Content,Purpose and Requirements of Functional Experiments Course1

1.1.1 Content1

1.1.2 Purpose1

1.1.3 Requirements1

1.1.4 Format of Experiment Report3

Section 2 Basic Knowledge Required in the Functional Experiments4

1.2.1 Basic Knowledge about Laboratory Animals4

1.2.2 How to Calculate the Drug Dosage to Animals11

1.2.3 Animal Handling and Different Routes of Administration12

1.2.4 Anesthesia of Laboratory Animals19

Section 3 Common Apparatus Used in Functional Experiments20

1.3.1 RM6240C Channel Physiological Signal Acquiring and Processing System20

1.3.2 BL-420 Data Acquisition and Analysis System for Life Science38

1.3.3 BI-2000 System of Image Processing and Analysis49

1.3.4 Computer and Local Network Technique Used in the Teaching51

CHAPTER TWO BASIC EXPERIMENTS IN FUNCTIONAL EXPERIMENTS53

Section 1 Analog Experiments on the Computer or Machine53

2.1.1 Analog Experiments of Electro-physiology of the Calamary's Huge Axon on the Computer53

2.1.2 Analog Experiments of Mechanics of Muscle Contraction on the Computer55

2.1.3 Analog Experiments of One Compartment Open Model on the Machine58

2.1.4 Analog Experiments of Two Compartment Open Model on the Machine60

Section 2 Functional Experiments on Isolated Preparations63

2.2.1 Preparation of Sciatic Nerve-Gastrocnemius(SNG)Samples63

2.2.2 Influences of the Muscle Contraction through Different Stimulation Intensities64

2.2.3 Influences of the Muscle Contraction through Different Stimulation Frequencies65

2.2.4 Preparation of Isolated Toad Heart66

2.2.5 Observation and Recording of Toad's Heart Beat68

2.2.6 The Effects of Electrolyte on the Heart Activity69

2.2.7 The Physiological Characters of the Rabbit Small Intestine Segment in-vitro70

2.2.8 The Influence of Drugs on Isolated Toad Heart72

2.2.9 Effect of Drugs on Receptors in Isolated Intestine Smooth Muscle of a Rabbit75

Section 3 Functional Experiments on Intact Preparations78

2.3.1 The Discharge of Rabbit Depressor Nerve78

2.3.2 Factors that Affect the Urine Formation in a Rabbit80

2.3.3 Comparing the Effects of Pentobarbital Sodium on Mice by Different Routes of Administration82

2.3.4 The Surface Anesthesia Effect of Tetracaine and Procaine on Rabbit Eyes83

2.3.5 The Epidural Anesthesia Effect of Procaine on Rabbits85

2.3.6 Mensuration Dose-effect Curve of NA on Blood Pressure in Rabbits86

2.3.7 Effect of Chlorpromazine on Body Temperature Regulation in Rats89

CHAPTER THREE INTEGRATED AND ADVANCED EXPERIMENTS IN FUNCTIONAL EXPERIMENTS90

Section 1 Multi-physiological Index Observation on Animal Body90

3.1.1 Nervous and Humoral Regulation of Arterial Blood Pressure in a Rabbit90

3.1.2 The Stimulus Effects on the Motor Area in Pallium of a Rabbit93

3.1.3 Nervous Regulation of the Respiratory Movement in a Rabbit94

3.1.4 Mensuration of ED50 of Sodium Pentobarbital in Mice95

3.1.5 Effects of Drugs on Cardiovascular System of a Rabbit98

Section 2 Preparation of Animal Disease Model100

3.2.1 A Rabbit Model for Hyperkalemia100

3.2.2 An Analysis of Experimental Edema in Toads103

3.2.3 Characterization of the Different Types of Hypoxia105

3.2.4 A Rabbit Model of Acute Hemorrhagic Shock107

3.2.5 The Effects of Ammonia on Hepatic Encephalopathy109

Section 3 Therapeutic Effects of Drugs on Animal Body111

3.3.1 Intoxication and Detoxication of Organophosphorus111

3.3.2 Anticonvulsant Effect of Drugs in Mice112

3.3.3 Observation of Analgesic Action of Drugs with Turning Trunk Method in Mice113

3.3.4 Toxic Effect of Streptomycin Sulfate and Antagonistic Effect of Calcium Chloride on a Rat115

3.3.5 Diuretic Effect of Drugs on a Rabbit116

3.3.6 Effect of Glucocorticoids on Permeability of Capillary Vessels in Mice118

CHAPTER FOUR EXAMINATION ITEMS IN FUNCTIONAL EXPERIMENTS119

Section 1 Observation and Preparation of Isolated Animal Samples119

4.1.1 Observation and Recording of Toad's Heart Beat119

4.1.2 Preparation of Sciatic Nerve-Gastrocnemius(SNG)Samples121

Section 2 Analysis and Preparation of Animal Disease Model122

4.2.1 A Rabbit Model of Acute Hemorrhagic Shock122

4.2.2 Nervous and Humoral Regulation of Arterial Blood Pressure in a Rabbit124

4.2.3 Factors that Affect the Urine Formation127

Section 3 An Analysis of Effects of Different Drugs on Animals or Samples129

4.3.1 Effect of Drugs on Receptors in Isolated Intestine Smooth Muscle129

4.3.2 Analysis of the Effects of Drags Acting on the Central Nerve System133

APPENDIX134

Preparation and Component of Experimental Solution134

Physiological Constants of Laboratory Animals134

REFEENCE135