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Chapter 1 Basics of Physiological-Pharmacological Experimentation1

Unit 1 Objectives and Requirements of Physiological-Pharmacological Experiment1

Unit 2 Process of Experiment Data and Production of Experiment Reports3

Unit 3 Requirements and Safety Rules in Laboratory7

Unit 4 Operation of Biological Data Acquisition System RM-624015

Chapter 2 General Knowledge of Physiological-Pharmacological Experimentation22

Unit 1 Fundamental Principle of Physiological-Pharmacological Experi-mentation22

Unit 2 Preliminary Tests in Physiological-Pharmacological Experimentation28

Unit 3 Statistical Analysis Most Often Used in Scientific Activities31

Unit 4 Statistic Analysis Using Excel35

Chapter 3 Basic Operations on Experimental Animals44

Unit 1 Fundamentals of Animal Experiments44

Unit 2 Operations on Experimental Animals48

Unit 3 Gender Identification and Labeling the Animals51

Unit 4 Narcotics and Animal Narcosis55

Unit 5 Sample Collection and Bleeding57

Unit 6 Drug Administration for Laboratory Animals60

Chapter 4 Physiological-Pharmacological Experiments66

Experiment 1 Generation and Conduction of Compound Action Potential66

Experiment 2 Actions of Antiepileptic and Anticonvulsants in Mouse Models70

Experiment 2-1 Effect of Drugs on Electric Convulsive Shock70

Experiment 2-2 Effect of Drugs on Convulsion Induced by Central Stimulants72

Experiment 3 Actions of Analgesics in Mouse Models74

Experiment 3-1 Analgesic Effect Assayed by Hot-Plate Model74

Experiment 3-2 Analgesic Effect Assayed Using Chemical-Stimulation Model76

Experiment 4 Influence of Stimulus on the Contraction of Skeletal Muscle78

Experiment 5 Determination of ABO Blood Group81

Experiment 6 Effects of Physical and Chemical Factors on Blood Coagulation83

Experiment 7 Recording of Electrocardiogram in Toads85

Experiment 8 Effects of Chemical Agents on the Activity of the Isolated Toad Heart88

Experiment 9 Regulation of Artery Blood Pressure91

Experiment 10 Measurement of Human Blood Pressure94

Experiment 11 Toxic Effects of Cardiac Glycosides on Heart97

Experiment 12 Effect of Drugs on Isolated Intestinal Smooth Muscles101

Experiment 13 Regulation of Respiratory Movements103

Experiment 14 Experimental Pulmonary Edema in Rabbits106

Experiment 15 Factors and Drugs Influencing Urine Output110

Experiment 16 Drug Actions Influenced by Dosage Forms and Delivery Routes113

Experiment 17 Determination of 50 3.485388E-317ffective Dose of Pentobarbital115

Experiment 18 Measurement of Concentration-Time Profile of Metronida-zole118

Experiment 19 Calculation of the Pharmacokinetic Parameters122

Experiment 20 Intoxication and Detoxification of Organophosphate Agents127

Experiment 21 Effect of Glucocorticoids on Intraabdominal Capillary Permeability in Mice130

Experiment 22 Effect of Anti-inflammatory Agents on Tumescence in Rat Model133

Experiment 23 In-Vitro Experiment of Antibacterial Actions136

Chapter 5 Expanded Experiments139

Experiment 1 Extraction of Genomic DNA in Peripheral Blood139

Experiment 2 Extraction of mRNA from Liver Tissues142

Experiment 3 Assessment of Gene Transcription Level by Real-Time PCR145

Experiment 4 Assessment of Protein Expression by Western-Blot148

Experiment 5 Isolation and Culture of Mouse Hepatocytes152

Appendix156

Table 1 Physiological parameters of experimental animals156

Table 2 Age,body weight and lifetime of adult experimental animals157

Table 3 Formularies of commonly used saline solution157

Table 4 Bleeding methods for experimental animals158

Table 5 Safe and lethal bleeding volume of experimental animals158

Table 6 Scaling factors for cross-species dose calculation159

Table 7 Application route and dose of commonly used anaesthetics160

参考文献161